Dental Journals
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Examination of cytotoxicity and mutagenicity of AH26 and AH Plus sealers.
Miletic I, Jukic S, Anic I, Zeljezic D, Garaj-Vrhovac V, Osmak M. - Int Endod J. 2003 May;36(5):330-5.
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AIM:
To study in vitro the cytotoxic and mutagenic effects of AH26 and AH Plus.
METHODOLOGY:
Cytotoxic effects on Chinese hamster V79 cells were determined by counting viable cells following incubation with eluations of
AH26 and AH Plus. In one set of experiments, the materials were mixed, set for
1 h and then eluted with dimethyl sulphoxide (DMSO) for 1 h, 24 h and 7 days.
In the other set, AH26 and AH Plus were mixed and set for 1 h, 24 h and 7 days
in physiological saline then crushed and eluted in DMSO for 24 h. The cytotoxic
effects of these eluates were evaluated. Three concentrations were chosen to
examine the mutagenic effects of AH26 and AH Plus: 5.57, 16.7 and 55.7 microg mL(-1).
The structural chromosomal aberration analysis and micronucleus test were performed
on human lymphocytes according to standard procedures.
RESULTS:
Dose-response curves of cell survival were obtained. Both materials were shown
to be cytotoxic in doses larger than 55.7 microg mL(-1), except for AH26, after
7 days setting time. AH Plus was also shown to be toxic in concentrations of 16.7
microg mL(-1), except after 7 days setting time. Neither AH26 nor AH Plus induced
a significant increase of chromosomal aberrations or micronuclei induction at any
setting time or concentration.
CONCLUSION:
There was no mutagenicity found for
AH26 and AH Plus on human lymphocytes in highly controlled conditions in vitro.
Full Text
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